Uracil in formic acid hydrolysates of deoxyribonucleic acid

Uracil in formic acid hydrolysates of deoxyribonucleic acid.

1. When DNA is hydrolysed with formic acid for 30min. at 175 degrees and the hydrolysate is chromatographed on paper with propan-2-ol-2n-hydrochloric acid, in addition to expected ultraviolet-absorbing spots corresponding to guanine, adenine, cytosine and thymine, an ultraviolet-absorbing region with R(F) similar to that of uracil can be detected. Uracil was separated from this region and ident...

Conversion of uracil deoxyriboside to thymidine of deoxyribonucleic acid.

Most of the literature dealing with the participation of citrovorum factor and vitamin Blz in the formation and transfer of l-carbon intermediates stresses the donor compounds serine, glycine, and formate as precursors of the 5-methyl group of thymidine (2). Uracil deoxyriboside, first isolated in crystalline form by Dekker and Todd (3) in 1950, is known to arise as a result of the deamination ...

Treatment of Warts with Topical Formic Acid

Deoxyribonucleic acid-deoxyribonucleic acid hybridization assay for replication origin deoxyribonucleic acid of Escherichia coli.

Deoxyribonucleic acid (DNA)-DNA hybridization on nitrocellulose filters can be used to assay for replication origin DNA from Escherichia coli if the DNA attached to the filters is enriched for the replication origin sequences. Such DNA can be readily isolated from very rapidly growing cells. When low amounts of this DNA were attached to filters, radioactively labeled DNA from the replication or...

Deoxyribonucleic acid-deoxyribonucleic acid hybridization of R factors.

A new method of estimating deoxyribonucleic acid homology is presented and used to measure the homology between bacterial plasmids of different compatibility groups. Deoxyribonucleic acid of the P-group plasmid RP1, originally isolated in Pseudomonas aeruginosa, has considerable (50%) homology with an N-group plasmid of the Enterobacteriaceae.